Positive Control: Inpp5e+/+ 4T1 mouse breast carcinoma cell line (transfected with non-targeting Cas9 gRNA), treated with an inducer (unpublished)
Negative Control: Inpp5eem 4T1 cell line (CRISPR/Cas9 endonuclease mediated Inpp5e knock-out)
Passed. ABIN2704925 specifically recognizes Inpp5e in murine Inpp5e+/+ 4T1 cells upon induction aside from other unspecific bands.
Submitted by: Children's Hospital of Eastern Ontario Research Institute